畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (11): 2198-2204.doi: 10.11843/j.issn.0366-6964.2017.11.022

• 基础兽医 • 上一篇    下一篇

保育猪舍不同粒径悬浮颗粒物细菌群落组成的初步研究

鞠雷, 郭洪梅*, 朱术会, 李丛丛   

  1. 山东畜牧兽医职业学院, 潍坊 261061
  • 收稿日期:2017-05-04 出版日期:2017-11-23 发布日期:2017-11-23
  • 通讯作者: 郭洪梅,E-mail:sdmyghm@163.com
  • 作者简介:鞠雷(1981-),山东泰安人,讲师,硕士,主要从事猪病防治及猪场环境控制研究,E-mail:zhyjul@sina.com,Tel:0536-3086364
  • 基金资助:

    山东省现代农业产业技术体系生猪创新团队生产与环境控制岗位(SDAIT-08-09);潍坊市科技发展计划(2016GX045)

Preliminary Study on Bacterial Community Composition of Different Particle Size of Suspended Particulates in Weaning Pig House

JU Lei, GUO Hong-mei*, ZHU Shu-hui, LI Cong-cong   

  1. Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China
  • Received:2017-05-04 Online:2017-11-23 Published:2017-11-23

摘要:

通过高通量测序技术,初步研究保育猪舍不同粒径悬浮颗粒物细菌群落的组成。利用Andersen-6级撞击式空气微生物样品收集器,以5%的公绵羊血-琼脂培养基为采样介质收集保育猪舍气溶胶微生物,培养24 h后提取总DNA,扩增16S rDNA的V3-V4区,进一步采用Illumina MiSeq高通量测序研究保育猪舍不同粒径悬浮颗粒物细菌群落组成。各级采样样品共获得高质量序列301 065条,按照序列相似度97%的标准对高质量序列进行OTU的划分,得到2 104个OUT;通过α多样性分析,菌群丰富度由大及小依次为3_4s、3_2s、3_1s、3_6s、3_5s、3_3s,通过不同分析指数菌群多样性略有差异,但是多样性最高的为3_2s、3_5s,最低的为3_3s;菌落组成分析来看,细菌主要来自4个门、40个菌属,其中丰度最高的是变形菌门、厚壁菌门,总比例较高的菌属为变形菌门的不动杆菌属和丛毛单胞菌属,放线菌门的葡萄球菌属以及拟杆菌门的类香味菌属;利用UniFrac的PCoA分析3_1s、3_5s、3_4s、3_2s菌群相似度较高;3_6s、3_3s与其余样品菌群结构差异明显。可见,2.1~3.3 μm粒径悬浮颗粒细菌菌群丰富度最高,有14个菌属的细菌被报道对人和动物具有致病性,且各层级群落结构存在差异,相邻层级差别较大。

Abstract:

Bacterial community composition of different particle size of suspended particulates in weaning pig house was preliminary studied, by high-throughput sequencing. This study collected aerosol microbe in weaning pig house with 5% of the male sheep blood-AGAR culture medium for sampling medium, using Andersen-level 6 impact type air microorganism sample collector. The collected aerosol microbe was trained after 24 hours to extract DNA and amplify of the V3-V4 viriable regions of 16S rDNA. Afterwords, we analyzed bacterial community composition of different particle size of suspension particles in weaning pig house, by iⅡumina MiSeq high-throughput sequencing. We obtained 301 065 high quality series at all levels and identified 2 104 OTUs (operational taxonomical unit) based on the 97% similarity rule; By alpha diversity analysis, flora richness from big to small, in turn, was 3_4s, 3_2s, 3_1s, 3_6s, 3_5s and 3_3s, respectively. Flora diversity was slightly different through the analysis of the different index, but the highest diversity was from 3_2s, 3_5s, the lowest was from 3_3s; Flora composition was mainly composed of 4 phylum, 40 species. The richness of Proteobacteria and Firmicutes were the highest. Total percentage of Acinetobacter, Comamonas, Staphylococcus and Myroides were higher than others. Flora similarity of 3_1s, 3_5s, 3_4s and 3_2s were high through the PCoA (principal component analysis) based on UniFrac analysis. Difference of flora structure between 3_6s, 3_3s and the others was observed. The richness of bacterial flora which diameter of suspended particulate was between 2.1 and 3.3 μm was the highest. Fourteen species of bacteria had been reported pathogenic to people and animals. Community structure was different at all levels, and difference of adjacent level was great.

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